Scientists reveal the mechanism of skeletal muscle stem cell activation in muscle regeneration

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Figure 1: Proteomic distribution and text signaling explain the potential of the translation control system during the SC quiescence-to-action transition. An example of a process for studying the pathogens of SCs isolated FACS. SCs were listed from Pax7-nGFP mice. QSCs were listed from 0.5% PFA-perfused rats. FiSCs were listed from untreated rats. Injured ASCs (iASCs) were listed from 3 days 1.2% BaCl2 injured. Normal ASCs (cASCs) are 2-day SCs (n = 2 independent tests). b Collection protein expression (in the spectrum) for QSCs, fiSCs, iASCs, and cASCs. c Interaction map for cell cycle, stress, and specific activation of QSCs, fiSCs, iASCs, and cASCs. d Heatmap is a representative expression for fatty acid oxidation and glycolysis-related proteins of QSCs, fiSCs, iASCs, and cASCs. e Heatmap of representation information for converting protein / receptors of QSCs, fiSCs, iASCs, and cASCs. f, g Analysis of transcriptome and proteome on QSCs and cASCs (proteome comparisons) and iASCs. f Disperse the RNA and protein conversion factor comparing QSC to ASCs. The heat map shows the RNA and protein expression of the molecular representation in the low-precision scattering scheme. g Analysis of molecular enhancement from the lower right of f’s g: Profiler. G: Profiler uses hypergeometric testing to measure the importance of verbs in a list of molecular inputs. Credit: DOI: 10.1038 / s41467-022-28612-1

Researchers at the Hong Kong University of Science and Technology (HKUST) have discovered the potential role of protein in the production of skeletal muscle cells to repair muscle after injury, laying the foundation for further research into developmental pathways. virus-to-virus. -repaired activation and regeneration of muscle-based tissue.

Muscle cramps, or satellite cells (SCs), are indispensable for repairing damaged muscles and are an important target for treating muscle diseases. In a healthy muscle that is not injured, these root canal cells are lying in it worry, Sleep state, to maintain a stable seat for future muscle repair. When muscle damage occurs, these tiny muscle cells will “wake up” quickly, “providing enough muscle tissue to build new muscle.

Despite being an important step muscle recoverya simple light-to-activate cell is a modern process, and scientists’ understanding of chemical systems – they are completely limited.

Recently, using all the mouse rotation techniques developed in its laboratory to obtain real-time quiescent SCs for low-input values, the scientific team at HKUST revealed that a protein called CPEB1 contributes to the transformation of the translation environment in SCs, thus expelling cells to activation and enhancement.

“In our study, we found a mismatch between the SC proteome and the text during activation, indicating the existence of the theory behind the writing,” said Professor Tom Cheung, team leader of research and SH Ho Assistant Professor of Life Sciences at HKUST. . “Our study shows that the protein levels of CPEB1 are not low in SCs, but they are normalized in activated SCs, with loss of CPEB1 delaying SC activation.”

In subsequent immunoprecipitation studies of RNA and CPEB1-knockdown proteomic analysis, the researchers found that CPEB1 phosphorylation modulates the expression of the myogenic factor MyoD – a protein involved. skeletal muscle progress – by targeting a series of sequences obtained in three unstructured regions (3’UTR) of the RNA transcription target to drive SC activation.

The results of their research were recently published online in the journal Environmental communication on February 17, 2022.

“It means that the use of CPEB1 or phosphorylation levels may increase SC production to produce sufficient myogenic progenitor cells for muscle repair, which may be the target of therapeutic treatment for muscle repair in the elderly,” said Professor Cheung, added. that the study will be. they play an important role in the field as scientists continue to conduct detailed research on cell proliferation and cell repair.

The next step of the group study would be to evaluate vivo muscle resuscitation in vivo in CPEB1-knockout mice to further strengthen the role of CPEB1 in SC-mediated skeletal muscle recovery. “Furthermore, by using high-performance screening, we can identify compounds that can modulate CPEB1 protein expression to enhance muscle recovery,” said Professor Cheung.


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Learn more:
Wenshu Zeng et al, CPEB1 directs the activation of the skeletal muscle by redesigning the translational structure, Environmental communication (2022). DOI: 10.1038 / s41467-022-28612-1

hint: Scientists describe skeletal muscle stimulation system in muscle regeneration (2022, February 24) retrieved February 24, 2022 from https://medicalxpress.com/news/2022-02-scientists-reveal-mechanism- skeletal-muscle.html

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Scientists reveal the mechanism of skeletal muscle stem cell activation in muscle regeneration Source link Scientists reveal the mechanism of skeletal muscle stem cell activation in muscle regeneration

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